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You are here:Home » Antibodies » Abs to Golgi Proteins » Anti -PIST (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL, dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Protein, GOPC, GOPC1, OT

Anti -PIST (PDZ Protein Interacting Specifically with TC10, CFTR-associated ligand, CAL,
dJ94G16.2, Fused in Glioblastoma, FIG, Golgi-associated PDZ and Coiled-coil Motif
Containing, Golgi Associated PDZ and Coiled Coil Motif Containing Protein, GOPC, GOPC1, OT

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #P4207-52
DescriptionAutophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be
defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl- 2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1 (6).
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug’ml
Optimal dilutions to be determined by the researcher.
Positive ControlPC-3 cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide PIST 17aa near the center of human PIST
(Genbank accession No. AAG00572).
SpecificityRecognizes human PIST. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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