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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Plectin

Anti -Plectin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified B IH
Plectin, a 500kD intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).
Catalog #P4214-32
Plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes).
ApplicationsSuitable for use Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 2ug/ml
Immunohistochemistry (Frozen): 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D75
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS.
PurityPurified by Protein G affinity chromatography. 95% by SDS-PAGE.
SpecificityRecognizes human plectin. Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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