Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Growth Factors, Cytokines » Growth Factors-Neurotrophins » Pleiotrophin, Recombinant, Human (PTN, HARP, Heparin Affin Regulatory Peptide)

Pleiotrophin, Recombinant, Human (PTN, HARP, Heparin Affin Regulatory Peptide)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Pleiotrophin (Osteoblast-Specific Factor-1, OSF-1) contains 136 amino acid residues. The sequence is very rich in cationic amino acids (24% of the residues); lysine cluster sequences are found in the Nterminal and C-terminal ends of the structure. The OSF-1 gene was shown by Northern blotting analysis to be expressed in mouse calvarial osteoblast-enriched cells and in mouse brain tissues, but not in thymus, spleen, kidney, liver, lung, testis or heart. Pliotrophin has the ability to promote adhesion, migration, expansion, and differentiation of human osteoprogenitor cells. In addition to certain types of cancer, the embryonic growth and differentiation factor pleiotrophin is found also in adults in inflammatory diseases. In osteoarthritis, pleiotrophin is especially expressed in early stages, and its concentrations in the synovial fluid could serve as a marker for the progress of the disease. Pleitrophin might be involved in cartilage repair in osteoarthritis, in particular, in earlier stages.
Catalog #P4214-50B
DescriptionRecombinant Human Pleiotrophin contains His-Tagged Fusion Protein, produced in E. coli, its molecular weight is 17.3kD protein containing 136 amino acid residues of the human OSF-1 and 16 additional amino acid residues-HisTag, thrombin cleavage site .
Amino Acid SequenceMKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD
SpecificityThe amino acid sequence of the recombinant human OSF-1 is 100% homologous to the amino acid sequence of the human OSF-1 without signal sequence.
ApplicationsWestern blotting, ELISA
SolubilityAdd 0.2 ml of PBS pH 7.2 and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
StabilityThe lyophilized protein remains stable until the expiry date when stored at -20°C.
Molecular Weight17.3kD
SourceE. coli
Purity95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in 0.1M phosphate buffer, 0.1M sodium chloride, pH 7.2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links