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You are here:Home » Antibodies » Abs to Plakophilin » Anti -Plakophilin 1

Anti -Plakophilin 1


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IF
Desmosomes are adhesive intercellular junctions that act as cell surface attachment sites for intermediate filaments and play a role in providing strength to many tissues. In epithelial cells, the cytoplasmic side of desmosomes, the ‘desmosomal plaque’, represents a specific attachment structure for the anchorage of intermediate filaments (IF) of the cytokeratin type. Plakophilin-1 protein is one of several proteins that make up the desmosomal plaque, including plakoglobin and desmoplakin 1 and 2.
Catalog #P4230-01
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunofluorescence: 3-10ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation (Native): 2ug/IP Reaction
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3G250
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenN-terminus of Human plakophilin-1.
SpecificityRecognizes the N-terminus of human plakophilin-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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