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You are here:Home » Antibodies » Abs to Plakophilin » Anti -Plakophilin 1

Anti -Plakophilin 1

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IF
Desmosomes are adhesive intercellular junctions that act as cell surface attachment sites for intermediate filaments and play a role in providing strength to many tissues. In epithelial cells, the cytoplasmic side of desmosomes, the ‘desmosomal plaque’, represents a specific attachment structure for the anchorage of intermediate filaments (IF) of the cytokeratin type. Plakophilin-1 protein is one of several proteins that make up the desmosomal plaque, including plakoglobin and desmoplakin 1 and 2.
Catalog #P4230-01
ApplicationsSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunofluorescence: 3-10ug/ml
Western Blot: 1-3ug/ml
Immunoprecipitation (Native): 2ug/IP Reaction
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 cell lysates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G250
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenN-terminus of Human plakophilin-1.
SpecificityRecognizes the N-terminus of human plakophilin-1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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