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You are here:Home » Antibodies » Abs to Plakophilin » Anti -Plakophilin 3

Anti -Plakophilin 3

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC
Several major families of cell adhesion molecules can be distinguished. These include the cadherins and N-CAMs, which play a crucial role in cell recognition and adhesion, while integrins are implicated in the adherence of cells to the extracellular matrix. Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. NCAMís (neural cell adhesion molecules) exist in at least three forms of plasma membrane plycoproteins, which are expressed in a variety of tissues including most nerve cells. The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an a- and a beta-subunit in interconnecting the cytoskeleton and the extracellular matrix.
Catalog #P4230-13
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionImmunocytochemistry: Methanol and paraformaldehyde-fixed cells.
Immunohistochemistry (Frozen and paraffn embedded): 1:25-1:200 (ABC)
Western Blot: 1:100-1:1000
Note: Pretreatment with 0.2% Triton-X 100 for 5 minutes for fixed cells and 15 minutes for frozen tissues, prior to incubation with primary antibody.
Optimal dilutions to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No4A82
HostMouse
SourceHuman
Concentration1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa570-589, CFTPQSRRLRELPLAADALTF of human plakophilin 3 (KLH via an additional cysteine at the N-terminus).
SpecificityRecognizes human Plakophilin 3. Species Crossreactivity: mouse and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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