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You are here:Home » Antibodies » Antibodies-Plakophilin » Anti -Plakophilin-3

Anti -Plakophilin-3


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IF
Plakophilin-3 is a novel 87kD Armadillo-like (Arm) protein, which is characterized by a series of repeats of 42-45 a.a., originally described in the Drosophila protein Armadillo. Plakophilin-3 is a member of the p120ctn/plakophilin subfamily, which includes plakophilin-1, plakophilin-2, the ARVCF gene product and d-catenin. This protein is similar to plakophilin-1 and plakophilin-2. Other Arm proteins include b-catenin and a-catenin. All these proteins are part of the cell-cell adhesion complex . On Western Blot analysis, plakophilin-3 was expressed in epithelial cell lines but not in foreskin fibroblasts and sarcomaderived cell lines. By Immunofluorescence, plakophilin-3 was colocalized with desmoglein in desmosomes of epithelial cells
Catalog #P4230-14
ApplicationsSuitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended DilutionELISA: 0.1-1ug/ml
Immunoprecipitation: 10ug/IP reaction
Immunohistochemistry: 10ug/ml
Immunoflluorescence: 1-3ug/ml
Western Blot: 1-3ug/ml
Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2% Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6A161
FormSupplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide
PurityPurified by Protein A chromatography
ImmunogenSynthetic peptide from Armadillo domain of human Plakophilin-3.
SpecificityReacts with the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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