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You are here:Home » Growth Factors, Cytokines » Growth Factors-PDGF » Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)

Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)

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Specifications

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kD disulfide-linked homodimer of two A chains (250aa).F.
Catalog #P4258
Biological ActivityDetermined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells. The expected ED50 for this effect is 1ng/ml.
Concentration Range 0.2-10ng/ml for most in vitro applications.
AA SequenceSIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
Storage and StabilityLyophilized powder may be stored at -20°C Stable for 12 months. Reconstitute with 10mM acetic acid. Aliquot and store at -20°C. Reconstituted product is stable for 3 months with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin USA
Also AvailableP4258: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01A: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-03: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) Mab Mo xHu
P4258-04: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-04A: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-14: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-16A: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-19: Platelet Derived Growth Factor AA,AB,BB Neutralizing (PDGF-AA,AB,BB) Pab Gt xHu
Molecular Weight28.5kD
SourceHuman, recombinant expressed in E. coli
Purity98% by SDS-PAGE and HPLC Endotoxin
FormSupplied as a lyophilized powder.. Reconstitute with 10mM acetic acid to 0.1-1mg/ml. This solution can then be diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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