| PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kD disulfide-linked homodimer of two A chains (250aa).F. |
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| Catalog # | P4258 |
| Biological Activity | Determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells. The expected ED50 for this effect is 1ng/ml. |
| Concentration Range | 0.2-10ng/ml for most in vitro applications. |
| AA Sequence | SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT |
| Storage and Stability | Lyophilized powder may be stored at -20°C Stable for 12 months. Reconstitute with 10mM acetic acid. Aliquot and store at -20°C. Reconstituted product is stable for 3 months with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Country of Origin | USA |
| Also Available | P4258: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) |
| P4258-01: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) |
| P4258-01A: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) |
| P4258-03: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) Mab Mo xHu |
| P4258-04: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu |
| P4258-04A: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu |
| P4258-14: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu |
| P4258-16A: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu |
| P4258-19: Platelet Derived Growth Factor AA,AB,BB Neutralizing (PDGF-AA,AB,BB) Pab Gt xHu |
| Molecular Weight | 28.5kD |
| Source | Human, recombinant expressed in E. coli |
| Purity | 98% by SDS-PAGE and HPLC Endotoxin |
| Form | Supplied as a lyophilized powder.. Reconstitute with 10mM acetic acid to 0.1-1mg/ml. This solution can then be diluted into other aqueous solutions. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
