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You are here:Home » Growth Factors, Cytokines » Growth Factors-PDGF » Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)

Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)

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Specifications

Catalog #P4258-01B
SourceRecombinant corresponding to human Platelet Derived Growth Factor AA expressed in E. coli.
Molecular Weight~28.5kD
Endotoxin ~0.1ng/ug
Biological ActivityED50 range=1-3ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application. Platelet derived growth factor is a mitogen for a variety of cell types, including connective tissue, smooth muscle tissue and fibroblasts.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% BSA, aliquot and store at-20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight28.5kD
SourceE. coli
Purity~98% (SDS-PAGE, HPLC)
ConcentrationNot determined
FormSupplied as a lyophilized powder, carrier free. Sterile filtered prior to lyophilization. Reconstitute in sterile ddH2O, 0.1% BSA to 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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