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You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)

Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)

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Specifications

Recombinant human platelet derived growth factor AA is a homodimeric, non-glycosylated, polypeptide chain containing 2x125aa and having a total molecular weight of 28.5kD. The sequence of the first five N-terminal amino acids was determined to be Ser-Ile-Glu-Glu-Ala. Contains less than 1% dimers and aggregates.
Catalog #P4258-01E
Source
E. coli
Molecular Weight~28.5kD
Biological ActivityThe ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is <1ng/ml, corresponding to a specific activity of 1MIU/mg.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceE. coli
Purity97% (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
FormSupplied as a lyophilized powder. Reconstitute using sterile deionized water to a concentration 100ug/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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