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You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

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Specifications

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-BB is a 24.3kD B chain homodimer protein containing 109 amino acid residues.
Catalog #P4258-15
Biological Activity The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is 1-3ng/ml.
Responding Cells (partial list) Fibroblasts, connective tissue
Concentration Range for most in vitro applications 0.2-10ng/ml
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, carrier protein. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight24.3kD
SourceHuman, recombinant protein expressed in E. coli
Purity98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug.
FormSupplied as a lyophilized powder from 10mM Acetic acid. No preservatives added. Reconstitute with ddH2Oand protein carrier to 0.1-1mg/ml. This solution can be diluted into other aqueous buffers.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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