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You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)

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Specifications

Recombinant protein expressed in yeast (S. cerevisiae) from the c-sis oncogene.
Catalog #P4258-15C
Sterility Sterilized through a 0.2um lter and packaged aseptically.
Reconstitution Dilution with 4mM HCl or 10mM acetic acid containing 1-2mg/ml BSA is recommended.
Note: In dilute solution, PDGF adheres to surfaces, particularly glass. Therefore, use only polypropylene containers, pipettes and tips. Biological activity is destroyed upon reduction.
Biological Activity 5ng/ml of this lot of PDGF-BB supported a 1.9-fold increase in cell growth of NIH 3T3 broblasts over control cultures without PDGF-BB. The optimal PDGF concentration in cell culture can vary depending on cell type.
PDGF Growth Assay1. Prepare a cell suspension in 2% platelet poor serum to a nal concentration of 1x10e4 cells/ml. Aliquot 4ml/tube to seed quadruplicate wells (1ml/well) of test, positive and negative controls.
2. Prepare PDGF-BB following instructions above for a concentration of approximately 1ug/ml and appropriate dilutions.
3. Add PDGF-BB directly to the 4ml suspension, mix well and dispense 1ml/well ( nal concentration 0-10ng/ml).
4. Refeed on day 3 with growth medium + PDGF-BB.
5. Count and record cell numbers on day 5. Calculate the stimulation factor over negative control results.
Storage and Stability Lyophilized powder may be stored at -20°C. May be stored at 4°C for short-term only. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight24.3kD
SourceYeast
PurityPuri ed by HPLC. SDS-PAGE Analysis: Reduced PDGF-BB runs as one band at 16-17kDa. Non-reduced PDGFBB runs as one band at 30-32kDa. Because of anomalous behavior, PDGF-BB sometimes runs as a doublet at 14-18kDa.
Form10ug PDGF-BB, lyophilized from 40ul of 0.1M acetic acid. Quantitated by amino acid analysis.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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