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You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB)

Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB)

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Specifications

Catalog #P4258-15M
SourceRecombinant corresponding to mouse Platelet Derived Growth Factor BB expressed in E. coli.
Molecular Weight~25kD
Endotoxin ~0.1ng/ug
Biological ActivityED50 range=1-5ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 100mM acetic acid, 0.1% BSA, aliquot and store at - 20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~25kD
SourceE. coli
Purity~95% (SDS-PAGE). Endotoxin: 0.1ng/ug.
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder, carrier free. Reconstitute in 100mM acetic acid, 0.1% BSA to 0.1-1mg/ml to regain full activity. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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