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Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB)
Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB)
Pricing
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| Catalog # | P4258-15M | | Source | Recombinant corresponding to mouse Platelet Derived Growth Factor BB expressed in E. coli. | | Molecular Weight | ~25kD | | Endotoxin | ~0.1ng/ug | | Biological Activity | ED50 range=1-5ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 100mM acetic acid, 0.1% BSA, aliquot and store at - 20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~25kD | | Source | E. coli | | Purity | ~95% (SDS-PAGE). Endotoxin: 0.1ng/ug. | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder, carrier free. Reconstitute in 100mM acetic acid, 0.1% BSA to 0.1-1mg/ml to regain full activity. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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