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You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB, Platelet-derived Growth Factor B Chain, Platelet-derived Growth Factor beta Polypeptide, Platelet-derived Growth Factor Subunit B, PDGF Subunit B, PDGFB, Becaplermin, FLJ12858, PDGF2, PDGF-2,

Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB, Platelet-derived Growth
Factor B Chain, Platelet-derived Growth Factor beta Polypeptide, Platelet-derived Growth
Factor Subunit B, PDGF Subunit B, PDGFB, Becaplermin, FLJ12858, PDGF2, PDGF-2,

Pricing

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Specifications

Catalog #P4258-17M
SourceRecombinant corresponding to 109aa of human Platelet Derived Growth Factor-BB is a homodimeric, glycosylated, polypeptide chain.
Molecular Weight~32kD
Biological ActivityThe ED50, calculated by the dose-dependent proliferation of mouse BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake).
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~32kD
Purity~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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