Platelet Derived Growth Factor CC, Recombinant, Human (PDGF-CC)
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| The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14kD polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR- and PDGFR- , and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet -granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF-R receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-R receptor. Recombinant human PDGF-CC is a 25kD protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains. | | | Catalog # | P4258-18H | | Amino Acid Sequence | MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG | | Biological Activity | Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50 for this effect is 15-20ng/ml. | | Storage and Stability | Lyophilized powder stored at -20°C is stable for 12 months. Reconstituted product is stable for 3 months when stored at -20°C in working aliquots with a carrier protein. Avoid freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Country of Origin | USA | | Source | E. coli | | Purity | 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1 ng/ug | | Concentration | As reported | | Form | Supplied as a lyophilized powder. Reconstitute with dH2O to 0.1-1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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