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You are here:Home » Molecular Biology » Growth Factors-PDGF » Platelet Derived Growth Factor CC, Recombinant, Human (PDGF-CC)

Platelet Derived Growth Factor CC, Recombinant, Human (PDGF-CC)

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Specifications

The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14kD polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR- and PDGFR- , and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet -granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF-R receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-R receptor. Recombinant human PDGF-CC is a 25kD protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains.
Catalog #P4258-18H
Amino Acid SequenceMVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG
Biological ActivityDetermined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50 for this effect is 15-20ng/ml.
Storage and StabilityLyophilized powder stored at -20°C is stable for 12 months. Reconstituted product is stable for 3 months when stored at -20°C in working aliquots with a carrier protein. Avoid freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
SourceE. coli
Purity98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1 ng/ug
ConcentrationAs reported
FormSupplied as a lyophilized powder. Reconstitute with dH2O to 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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