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You are here:Home » Antibodies » Abs to Plexin, Semaphorin » Anti -Plexin A4

Anti -Plexin A4

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B
Plexin A4 is a 195kD member of the plexin A subfamily, plexin family of proteins. It is found on sensory, autonomic and motor neurons, plus T and dendritic cells. Plexin A4 negatively regulates cell migration, activation and axon guidance, and does so by serving as a receptor for Sema6A and 6B, and as a co-receptor with neuropilin-1 and -2 for Sema3A. Mature human Plexin A4 is an 1871aa type I transmembrane glycoprotein. It contains a 1214aa extracellular domain (ECD) and a 636aa cytoplasmic region. The ECD contains one Sema-domain (aa51-482), three PSI domains (aa509-856) and four IPT regions (aa858-1230) that contain a phosphoserine at #946. There are three isoform variants. One shows a 65aa substitution for (aa458-1894), a second shows an 80aa substitution for (aa1292-1894), and a third shows the just mentioned 80aa substitution coupled to a 14aa substitution for (aa1-535). Over (aa24-1237), human Plexin A4 is 97% aa identical to mouse Plexin A4.
Catalog #P4271-17
ApplicationsSuitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1.0ug/ml. Detection limit is ~2.5ng/well for rhPlexin A4.
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceGoat
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography
ImmunogenRecombinant protein corresponding to aa24-1237 from human Plexin A4 expressed in CHO cells (Q9HCM2).
SpecificityRecognizes human Plexin A4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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