Anti -Plexin B1 (PLXNB1, KIAA0407, MGC149167, Plexin-B1, PLEXIN-B1, PLXN5, Semaphorin
Receptor SEP, SEP)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E FC |
|
| Plexin B1 is a 300kD member of the B subfamily, plexin family of semaphorin receptors. Mature human Plexin B1 is a 2116aa type I transmembrane (TM) glycoprotein that contains a 1471aa extracellular domain (ECD) and a 612aa cytoplasmic region. The ECD contains one semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200kD alpha-chain (aa20-1305) and a 100kD TM beta-chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. PlexinB1 is a receptor for CD100/Sema4D. It forms a receptor complex with neuropilins, MET, and EGFR2. Multiple splice variants are known. One shows a deletion of aa689-871. A second shows a 53aa substitution for aa677-2135. And a third shows a 53aa substitution for the 53aa between aa677-729. over aa35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively. | | | Catalog # | P4271-28 | | Applications | Suitable for use in ELISA and Flow Cytometry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 25ug/ml, 10ul labels 1-2.5 x 10e5 cells in 100-200ul | | Optimal dilutions to be determined by the researcher. | | Hybridoma | Mouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human Plexin B1 (aa35-150). | | Storage and Stability | Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 9L849 | | Host | Mouse | | Source | Human | | Concentration | As reported | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified, E. coli-derived, recombinant human Plexin B1 (aa35-150; Accession # O43157). | | Specificity | Recognizes human Plexin B1. Species sequence homology: canine (90%), mouse (97%). | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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