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P4271-28 Mouse Anti-Plexin B1 (PLXNB1, KIAA0407, Plexin-B1, PLEXIN-B1, PLXN5, Semaphorin Receptor SEP, SEP)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
O43157
Isotype
IgG1
Clone Number
9L849
Grade
Affinity Purified
Applications
FC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Plexin B1 is a 300kD member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200kD alpha -chain (aa 20-1305) and a 100kD TM beta -chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677‑2135, and a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with canine and mouse Plexin B1, respectively.

Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 2.5ug/10e6 cells in jurkat human acute T cell leukemia cell line Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
NS0 mouse myeloma cell line transfected with human Plexin B1
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human Plexin B1.
Conjugates
Pricing
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