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You are here:Home » Antibodies » Abs to Plexin, Semaphorin » Anti -Plexin B1 (PLXNB1, KIAA0407, MGC149167, Plexin-B1, PLEXIN-B1, PLXN5, Semaphorin Receptor SEP, SEP)

Anti -Plexin B1 (PLXNB1, KIAA0407, MGC149167, Plexin-B1, PLEXIN-B1, PLXN5, Semaphorin
Receptor SEP, SEP)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E FC
Plexin B1 is a 300kD member of the B subfamily, plexin family of semaphorin receptors. Mature human Plexin B1 is a 2116aa type I transmembrane (TM) glycoprotein that contains a 1471aa extracellular domain (ECD) and a 612aa cytoplasmic region. The ECD contains one semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200kD alpha-chain (aa20-1305) and a 100kD TM beta-chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. PlexinB1 is a receptor for CD100/Sema4D. It forms a receptor complex with neuropilins, MET, and EGFR2. Multiple splice variants are known. One shows a deletion of aa689-871. A second shows a 53aa substitution for aa677-2135. And a third shows a 53aa substitution for the 53aa between aa677-729. over aa35-150, human Plexin B1 is 90% and 97% aa identical to dog and mouse Plexin B1, respectively.
Catalog #P4271-28
ApplicationsSuitable for use in ELISA and Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 25ug/ml, 10ul labels 1-2.5 x 10e5 cells in 100-200ul
Optimal dilutions to be determined by the researcher.
HybridomaMouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human Plexin B1 (aa35-150).
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9L849
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified, E. coli-derived, recombinant human Plexin B1 (aa35-150; Accession # O43157).
SpecificityRecognizes human Plexin B1. Species sequence homology: canine (90%), mouse (97%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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