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You are here:Home » Antibodies » Abs to Plexin, Semaphorin » Anti -Plexin C1 (CD232, Virus-Encoded Semaphorin Protein Receptor, VESPR)

Anti -Plexin C1 (CD232, Virus-Encoded Semaphorin Protein Receptor, VESPR)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC
Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210kD type I transmembrane glycoprotein in the C subfamily of the large plexin family. It is widely expressed in neuronal and non-neuronal fetal and adult tissues. Plexin C1 is a receptor for the semaphorin homolog A39R encoded within poxvirus genomes, as well as the herpes virus Sema protein. The large Plexin C1 extracellular domain (ECD) contains one Sema-domain and two cysteine-rich Met-related sequences (MRS). In the region used as immunogen, human Plexin C1 shares 92% amino acid sequence homology with mouse Plexin C1.
Catalog #P4271-35
ApplicationsSuitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml; The detection limit is ~1ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 50ug/ml; Add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. Tested on white blood monocytes.
Direct ELISA: 0.5-1.0ug/ml; The detection limit is ~0.5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by Immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant human Plexin C1 (aa49-161), Accession #
SpecificityRecognizes human Plexin C1. In direct ELISAs and Western Blots, this antibody shows ~10% crossreactivity with recombinant mouse Plexin A1, A2, and A3, recombinant human Plexin B1, and <1% crossreactivity with recombinant human Plexin D1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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