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You are here:Home » Antibodies » Abs to Plexin, Semaphorin » Anti -Plexin D1

Anti -Plexin D1

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B FC
Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family. It is expressed on multiple tissues including fetal neuronal and endothelial cells. Plexin D1 expression is also induced on tumor vasculature and tumor cells. The 1879 amino acid (aa) mature human Plexin D1 contains a large (1225 aa) extracellular domain with a 500 aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs. By alternative splicing, multiple Plexin-D1 isoforms may exist. Plexin D1 binds Sema4A to suppress angiogenesis. It also binds Sema3E directly to mediate repulsive vascular vessal guidance. In mouse, this interaction has been shown to be independent of Neuropilin and Sema3A. Over the extracellular region used as immunogen, human Plexin D1 shares 89% and 91% aa identity with the mouse and canine protein, respectively.
Catalog #P4271-45
ApplicationsSuitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested.
Recommended DilutionFlow Cytometry: 25ug/ml. Add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane under non-reducing and reducing conditions.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.2ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by affinity chromatography.
ImmunogenNS0-derived recombinant human Plexin D1 extracellular domain (aa47-1271) Accession # Q9Y4D7
SpecificityRecognizes human Plexin D1. Species sequence homology: mouse 89% and canine 91%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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