| Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family. It is expressed on multiple tissues including fetal neuronal and endothelial cells. Plexin D1 expression is also induced on tumor vasculature and tumor cells. The 1879 amino acid (aa) mature human Plexin D1 contains a large (1225 aa) extracellular domain with a 500 aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs. By alternative splicing, multiple Plexin-D1 isoforms may exist. Plexin D1 binds Sema4A to suppress |
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| angiogenesis. It also binds Sema3E directly to mediate repulsive vascular vessal guidance. In mouse, this interaction has been shown to be independent of Neuropilin and Sema3A. Over the extracellular region used as immunogen, human Plexin D1 shares 89% and 91% aa identity with the mouse and canine protein, respectively. |
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| Catalog # | P4271-45A |
| Applications | Suitable for use in Western Blot and Flow cytometry. Other applications have not been tested. |
| Recommended Dilutions | Western blot: 0.1 0.2ug/ml. The detection limit is~1ng/lane under non-reducing and reducing conditions. |
| Flow cytometry: 25ug/ml. Add 10il of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of biotinylated antibodies may be visualized by adding a secondary developing reagent such as streptavidin conjugated to a fluorochrome. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Produced in a goat immunized with purified, NS0-derived, recombinant human Plexin D1 extracellular domain (rhPlexin D1; aa 47-1271). |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.05mg/ml |
| Form | Supplied as a lyophilized powder from PBS with BSA. Labeled with biotin. Reconstitute with 1ml TBS, pH7.3, containing 0.1% BSA to a concentration of 0.05mg/ml. |
| Purity | Purified by human Plexin D1 affinity chromatography. |
| Immunogen | NS0-derived recombinant human rhPlexin D1 extracellular domain. |
| Specificity | Recognizes human Plexin D1 extracellular domain. Species sequence homology: Mouse 89% and canine 91%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
