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You are here:Home » Molecular Biology » Growth Factors-Placenta, PlGF » PlGF2, Recombinant, Human (Placental Growth Factor 2)

PlGF2, Recombinant, Human (Placental Growth Factor 2)

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Specifications

Placenta growth factor (PlGF), a homodimeric glycoprotein that is homologous to vascular endothelial growth factor (VEGF), is mitogenic to endothelial cells and protects trophoblast from apoptosis. Alternative splicing of mature mRNA gives rise to three known isoforms of PlGF. PlGF is expressed by human trophoblast during normal pregnancy, however, it is not known which isoforms are produced.
Catalog #P4273-20
Recombinant Human PLGF-2 produced in insect cells is a homodimer comprised of glycosylated polypeptide chains containing 2 x 152 amino acids and having a total molecular mass of 44kD. Recombinant Human PLGF-2 is purified by proprietary chromatographic techniques
Biological ActivityBiologically active when compared to standard. Can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.3-10ng/ml corresponding to a specific activity of 0.1-3.4 MIU/mg.
Storage and StabilityLyophilized human PLGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -20°C. Upon reconstitution human PLGF-2 may be stored at 4°C for up to 7 days and for long-term storage, in aliquots at -20°C. Please avoid freeze-thaw cycles.
Molecular Weight44kD
SourceInsect cells
Purity80% as determined by RP-HPLC and SDS-PAGE
FormSupplied as a lyophilized powder containing BSA. Reconstitute in sterile 20mM acetic acid to a concentration of 100ug/ml, which can then be further diluted to other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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