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You are here:Home » Molecular Biology » MB-Serum Proteins » PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)

PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)

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Specifications

Mouse PLTP Phospholipid transfer protein (PLTP; also lipid transfer protein II) is an 80kD, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family of molecules (1). It is synthesized as a precursor with a 17aa signal sequence and a 476aa mature chain (SwissProt # P55065). The mature chain contains two BPI/LBP/CETP domains (aa21-239 and 263-463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4-6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha-tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7-11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6).
Catalog #P4278-01
SourceA DNA sequence encoding the mature mouse PLTP (Glu 18-Ala 493; AAH03782)
was fused with a 6X-His tag at the C-terminus and expressed in a mouse myeloma cell line, NS0.
Molecular MassBased on N-terminal amino acid sequencing, the recombinant mouse PLTP starts at Glu 18 and has a calculated molecular mass of approximately 53.5kD. The recombinant mouse PLTP migrates as an approximately 70-85kD protein in SDS-PAGE under reducing conditions.
ActivityMeasured by its ability to inhibit LPS-induced TNF-alpha secretion by Raw264.7 cells.
Storage and Stability12 months from date of receipt, 20 to 70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, 20 to 70°C under sterile conditions after reconstitution.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
PurityPurified 95% (SDS-PAGE). Endotoxin: 1.0 EU/1ug (LAL).
ConcentrationAs reported
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 50ug of BSA per ug of protein. Reconstitute with 40-50% glycerol, PBS.
SpecificityRecognizes mouse PLTP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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