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You are here:Home » Antibodies » Abs to Stem Cell Research » Anti -PlurES-1 (Pluripotent Human Embryonic Stem Cells-1)

Anti -PlurES-1 (Pluripotent Human Embryonic Stem Cells-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IC FC
Human embryonic stem cells (hESCs) have been traditionally characterized using a panel of antibodies to cell surface markers, such as SSEA-3, TRA-1-60, and TRA-1-81; however, none of these traditional markers are entirely specific to hES cells (Laslett et al., 2003). Therefore, there is a continuous quest to not only identify, but also gain a better understanding of new pluripotency markers to better characterize the human embryonic stem cell populations of interest. The novel hPlurES-1 Antibody is a monoclonal antibody that has been shown to identify a 65kD antigen that is expressed on the cell surface of human embryonic stem cells. This antibody recognizes a protein epitope that appears to be expressed within a subset of Oct-4-positive human embryonic stem cell populations, as indicated by double-staining analysis using flow cytometry and immunocytochemistry.
Catalog #P4284
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 25ug/ml
Western Blot: 1.5ug/ml
Immunocytochemistry: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H264
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. No preservatives added.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman Embryonic Stem Cells (hESCs).
SpecificityRecognizes a cell surface epitope of a 65kD protein found to be expressed on pluripotent human embryonic stem cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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