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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IF

Pma1p is the major plasma membrane H+-ATPase found in yeast. It plays a significant role in regulating intracellular ion homeostasis and is a useful marker of the plasma membrane.
Catalog #	P4285-35
Applications	Suitable for use in Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution	Western Blot (ECL): 1:15000
	Western Blot (Non-ECL): 1:500-1:5000
	Immunofluoscence: 1:250-1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	8A241
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Generated from sequence of yeast Pma1p sequence CAA96708.
Specificity	Recognizes yeast plasma membrane ATPase Pma1p. Reacts with Pma1p based on the size of the band detected on Western Blots and the Immunofluorescence localization pattern. Also detects Pma1p-containing fusion proteins on Western Blots.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.