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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -Pma1p

Anti -Pma1p

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IF
Pma1p is the major plasma membrane H+-ATPase found in yeast. It plays a significant role in regulating intracellular ion homeostasis and is a useful marker of the plasma membrane.
Catalog #P4285-35
ApplicationsSuitable for use in Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionWestern Blot (ECL): 1:15000
Western Blot (Non-ECL): 1:500-1:5000
Immunofluoscence: 1:250-1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No8A241
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenGenerated from sequence of yeast Pma1p sequence CAA96708.
SpecificityRecognizes yeast plasma membrane ATPase Pma1p. Reacts with Pma1p based on the size of the band detected on Western Blots and the Immunofluorescence localization pattern. Also detects Pma1p-containing fusion proteins on Western Blots.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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