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Protein Phosphatase Methylesterase, Recombinant, Human, aa1-386, His-tag (PME 1)
Protein Phosphatase Methylesterase, Recombinant, Human, aa1-386, His-tag (PME 1)
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| PPME1, also known protein phosphatase methylesterase-1(PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile. Purified by using conventional chromatography. | | | Catalog # | P4290-05C | | Source | Recombinant corresponding to aa1-386 of human PPME1, fused to His-tag at N-terminal, expressed in E.coli. | | AA Sequence | MGSSHHHHHH SSGLVPRGSH MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA | | IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ | | MQVLPQCGHA VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC | | Molecular Weight | ~44.4kD (406aa), confirmed by MALDI-TOF | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~44.4kD | | Source | E.coli | | Purity | ~95% (SDS-PAGE) | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH8, 0.1M sodium chloride,1mM DTT, 20% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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