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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » Protein Phosphatase Methylesterase, Recombinant, Human, aa1-386, His-Tag (PME 1)

Protein Phosphatase Methylesterase, Recombinant, Human, aa1-386, His-Tag (PME 1)

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Specifications

PPME1, also known protein phosphatase methylesterase-1(PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile. Purified by using conventional chromatography.
Catalog #P4290-05C
SourceRecombinant corresponding to aa1-386 of human PPME1, fused to His-tag at N-terminal, expressed in E.coli.
AA SequenceMGSSHHHHHH SSGLVPRGSH MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA
IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ
MQVLPQCGHA VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC
Molecular Weight~44.4kD (406aa), confirmed by MALDI-TOF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~44.4kD
SourceE.coli
Purity~95% (SDS-PAGE)
Concentration~0.5mg/ml
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH8, 0.1M sodium chloride,1mM DTT, 20% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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