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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -PNMT (Phenylethanolamine N Methyltransferase)

Anti -PNMT (Phenylethanolamine N Methyltransferase)

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum IH
Phenylethanolamine-N-methyltransferase (PNMT) is an enzyme converting noradrenaline to adrenaline. The enzyme is present in adrenomedullary cells and in the brain neurons.
Catalog #P4350-06
ApplicationsSuitable for use in Immunohistochemistry (frozen and paraffin sections). Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:400-1:800 (PAP) with overnight incubation at 4°C.
Optimal dilutions to be determined by the researcher.
Positive ControlDEPC-fixed paraffin sections of rat adrenal gland.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, dH2O. Reconstituted product is stable for 12 months at -20°C Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceBovine
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Reconstitute in 100ul sterile 40-50% glycerol, dH2O.
PuritySerum
ImmunogenPhenylethanolamine-N-methyltransferase from bovine adrenal medulla (Sigma).
SpecificityRecognizes bovine PNMT. Absorption with 10-100ug immunogen per ml diluted antiserum abolishes the staining. Species Crossreactivity: rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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