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You are here:Home » Antibodies » Abs to Enzymes, Phospholipase » Anti -PNPLA2 (PEDFR, Patatin-like phospholipase domain containing 2, 1110001C14Rik, Adipose triglyceride lipase, ATGL, Calcium-independent phospholipase A2, desnutrin, Desnutrin, DESNUTRIN, DKFZp667M109, FP17548, IPLA2-zeta, Patatin-like phospholipase domain-co

Anti -PNPLA2 (PEDFR, Patatin-like phospholipase domain containing 2, 1110001C14Rik,
Adipose triglyceride lipase, ATGL, Calcium-independent phospholipase A2, desnutrin,
Desnutrin, DESNUTRIN, DKFZp667M109, FP17548, IPLA2-zeta, Patatin-like phospholipase
domain-co

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IH
PEDF R (Pigment epithelium derived factor receptor; also ATGL, PNPLA2 and desnutrin) is a 55kD member of the PNPLA family of proteins. It is expressed in adipocytes, hepatocytes, skeletal muscle and pigment epithelium. PEDF R specifically hydrolyses triglycerides, releasing long-chain fatty acids. Mouse PEDF R is described as being a 486aa type II transmembrane
(TM) protein. Its TM segment is stated to span aa9-29. Alternatively, it may have a hydrophobic lipid-association domain between aa 267-295. It contains a palatin domain (aa10-179) with a conserved GxSxG enzymatic motif. There are multiple splice forms. Two show alternate start sites at Met57 and Met232, while two others show a deletion of aa163-196 and 253-308,
respectively. Over aa 62-253, mouse PEDF R shares 98% and 93% aa identity with rat and human PEDF R, respectively.
Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Catalog #P4350-15
Recommended DilutionWestern Blot: 1.0ug/ml
Immunohistochemistry: 5-15ug/ml detects PNPLA2 in cells and tissues
Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant mouse and recombinant human PNPLA2 is ~1ng/well
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa162-253 from mouse PNPLA2, expressed in E. coli (Q8BJ56).
SpecificityRecognizes human PNPLA2. Species crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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