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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IP

The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn21-dependent protease, unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs, and also to ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.
Catalog #	P4355-01
Applications	Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	Western Blot: 1-3ug/ml
	Immunoprecipitation (Mouse): 7ug/IP reaction
	Optimal dilutions to be determined by the researcher.
Positive Controls	HeLa and NIH3T3
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide derived from C-terminal region of POH1 (26S proteosome-associated pad1 homolog/ non-ATPase subunit also known as Rpn11 in yeast.
Specificity	Recognizes the human and mouse POH1 proteins at ~35kD.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.