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You are here:Home » Antibodies » Abs to Chromatin Proteins » Anti -Polycomb 2 (CBX4, Chromobox homolog 4 (Pc class homolog, Drosophila), Chromobox protein homolog 4, E3 SUMO-protein ligase CBX4, hPc2, hPC2, NBP16, Pc2, PC2, Polycomb 2 homolog)

Anti -Polycomb 2 (CBX4, Chromobox homolog 4 (Pc class homolog, Drosophila), Chromobox
protein homolog 4, E3 SUMO-protein ligase CBX4, hPc2, hPC2, NBP16, Pc2, PC2, Polycomb 2
homolog)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
C2 (CBX4, Cbx4: chromobox homolog 4 (Drosophila Pc class), Chromobox protein homolog 4, hPc2, Pc2, Polycomb 2 homolog) is directed against targets that have limited or no commercial antibodies available to them and for which there are no data on the expression of the protein in the range of common cell lines and tissues. These antibodies are affinity purified using their peptide immunogen and are known to give low background staining in a western blot. However no additional claims are made for their ability to recognize native protein in any application 'Polycomb' (Pc) and other members of the Drosophila Polycomb group gene family are part of a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. Pc shares a homologous domain known as the chromodomain with the Drosophila heterochromatin-binding protein, HP1. To isolate human Pc homologs, Satijn et al. (1997) screened a fetal brain cDNA library with a probe containing the nonchromodomain portion of a Xenopus Pc homolog (XPc). They recovered a cDNA encoding a predicted 558-amino acid protein that they designated human Pc2. Human Pc2 and XPc share 48% protein sequence identity. Northern blot analysis revealed that human Pc2 is expressed as a 2.8-kb transcript in all tissues tested with almost equal abundance. Using immunofluorescence, Satijn et al. (1997) found that human Pc2 colocalizes with another human Pc homolog, CBX2 (602770), in interphase nuclei, suggesting that the proteins are part of a complex.
Catalog #P4363-10
ApplicationsSuitable for use in Immunoprecipitation and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 2ug/ml
Optimal dilution to be determined by researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to the N-terminal, aa1-228 of human Polycomb 2.
SpecificityRecognizes human Polycomb 2. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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