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You are here:Home » Biochemicals » Biochem-Antibiotics » Polymyxin B (PMB) (HRP)

Polymyxin B (PMB) (HRP)

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Specifications

The peptide Polymyxin B (PMB) is a cyclic amphipathic peptide antibiotic that binds to endotoxins (lipopolysaccharide, LPS). Binding of PMB to LPS results in neutralization of LPS of gram-negative bacteria. For the study of PMB-LPS interactions PMB conjugates proved to be superior to the application of anti-PMB antibodies. PMB conjugates are useful general probes to detect or measure lipid A and LPS of various species using simple methods. These conjugates are also useful for staining of bacteria. The conjugates obviate the need for many specific antisera. Furthermore PMB conjugates are useful tools in investigations of LPS binding protein (LBP) and bactericidal permeability increasing protein (BPI) interactions with LPS. In conclusion, PMB conjugates are useful probes for endotoxin research.
Catalog #P4400-15A
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionsELISA: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
PurityPurified
ConcentrationNot determined.
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% 2-chloroacetamide. Labeled with Horseradish Peroxidase (HRP).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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