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You are here:Home » Antibodies » Abs to Glycols » Anti -Polyethylene Glycol (PEG) (Biotin)

Anti -Polyethylene Glycol (PEG) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified E B
Polyethylene glycol (PEG) is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution. The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs. The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions. As examples, PEG-adenosine deaminase (Adagen) is used for the treatment of severe combined immunodeficiency syndrome, PEG-asparaginase (Oncaspar) is used for the treatment acute lymphoblastic leukemia, PEG-interferon alpha 2a (Pegasys) is used for the treatment Hepatitis C, Branched PEG-anti-VEGF aptamer (Pegaptanib, Macugen) is used for the treatment Macular degeneration (age-related). An Anti-PEG antibody can be used to monitor a drugs pharmacokinetics, including distribution, metabolism and excretion.
Catalog #P4444-26
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.5ug/ml for use as a detection antibody. Use 5-10ug/ml to coat plates for use as capture antibody.
Western Blot: 15.6ng/ml (1:5000-1:20,000)
Optimal dilutions to be determined by the researcher.
Affinity Constant3.57e10L/mol
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E454
HostRabbit
Concentration~0.5mg/ml
FormSupplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenPEG with terminal methoxy group conjugated with KLH..
SpecificityRecognizes the terminal methoxy group of the PEG molecule. Recognizes various length Y-chain PEG molecules as well as single chain PEG molecules with equal affinity. Does not crossreact with non-specific targets in blood or serum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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