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Specifications

Clone Host Grade Applications Monoclonal Rabbit Affinity Purified E B IH

Polyethylene glycol PEG is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution (1). The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs (2,3). The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions (4). As examples, PEG-adenosine deaminase (Adagen®) is used for the treatment of severe combined immunodeficiency syndrome, PEG–asparaginase (Oncaspar®) is used for the treatment acute lymphoblastic leukemia, PEG–interferon alpha-2a (Pegasys®) is used for the treatment Hepatitis C, Branched PEG–anti-VEGF aptamer (Pegaptanib, Macugen™) is used for the treatment Macular degeneration(age-related) (5). An anti-PEG antibody can be used to monitor a drug’s pharmacokinetics, including distribution, metabolism and excretion. In addition, it can be used for the quality control of pegylated molecules in ELISA, Western Blot and Flow Cytometry. In both direct and sandwich ELISA assays, P4444-27 shows greater affinity and accuracy than other PEG antibodies when determining the concentration of PEG or PEG-modified proteins. Results were similar whether detecting PEG itself or PEG-modified targets.
Catalog #	P4444-27
Applications	Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: 200ng/ml-1ug/ml
	Immunohistochemistry: 1-5ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E454
Host	Rabbit
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Purity	Purified by Protein A affinity chromatography.
Immunogen	PEG with terminal methoxy group, conjugated to KLH.
Specificity	Recognizes the terminal methoxy group of the PEG molecule. Detects various length Y-chain PEG molecules as well as single chain PEG molecules with equal affinity. Does not cross react with non-specific targets in blood or serum.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.