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You are here:Home » Antibodies » Abs to CD Markers » Anti -Polysialic Acid NCAM (PSA-NCAM)

Anti -Polysialic Acid NCAM (PSA-NCAM)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IH IC RIA
Polysialic acid (PSA) is a long, linear homopolymer that is attached to the neural cell adhesion molecule (NCAM). It has the remarkable ability to down-regulate a wide variety of contact-dependent cell interactions. This activity is believed to stem from the ability of the charged polymer to occupy a large hydrated volume and thereby cause a direct physical hindrance of cell–cell contact. The regulated expression of PSA has been shown to promote a reduction in cell interactions that can create permissive conditions for changes in the structure of a variety of tissues.
The broadest expression of PSA occurs on precursor cells during early development and plays a role in promotion of the migration of these cells after cell division. In most cases, PSA expression is lost after the migration process is completed. However, the axons of neurons often retain PSA to bundle, sprout, and branch appropriately during axon path-finding.
Catalog #P4460
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and RIA. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:500-1:1000
Immunocytochemistry: 1:200-1:400; Works on live and fixed cells.
Immunohistochemistry: 1:200-1:400; Works on tissue sections either frozen or fixed with any kind of fixative.
Immunohistochemistry (Paraffin): 1:100. Requires retrieval with EDTA, pH 8.0. Note: Variable results have been obtained on paraffin sections.
Optimal dilutions to be determined by the researcher.
Recommended Control TissueYoung rat hippocampus, human hippocampus
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgM
Clone No2Q130
HostMouse
SourceMouse
ConcentrationNot Determined
FormSupplied as a liquid in 50% glycerol. No preservatives added.
PurityAscites
ImmunogenViable Meningococcus group B (strain 355) (NM_000615.5).
SpecificityRecognizes mouse Polysialic Acid NCAM at 150-300kD. Works well on all vertebrate species expressing PSA-NCAM.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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