Anti -POU Domain, Class 2, Transcription Factor 1 (POU2F1, POU Class 2 Homeobox 1, NF-A1,
Octamer-binding Transcription Factor 1, OCT1, OCT-1, OTF1, OTF-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Ascites |
B IP Gel |
|
| OCT1 is a transcription factor that binds the octamer motif 5’-ATTTGCAT-3’ in promoters of various genes.OCT1 contains one homeobox DNA binding domain and is part of the POU transcription factor family due to the presence of one POU domain, a highly charged motif that selectively binds the octamer motif.OCT1 is also known as POU domain class 2 transcription factor, octamer-binding transcription factor, oct-1, OTF-1, NF-A1, and POU2F1. | | | Catalog # | P5250-01D | | Applications | Suitable for use in Western Blot, Gel Shift Binding and Immunoprecipitation. Other applications have not been tested. | | Recommended Dilution | Western Blot: 1:250 -1:1000 | | Immunoprecipitation: 20ul | | Gel Shift Binding: 2ul of this antibody will supershift an Oct-1/DNA complex. | | Optimal dilutions to be determined by the researcher. | | Recommended secondary antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo | | Positive Antigen Control | (H1838) | | HeLa nuclear extract in RIPA buffer. Add an equal volume of Laemmli reducing sample buffer to 10ul of extract and boil for five minutes to reduce the preparation. Use 20ug per lane for minigels. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 0.T.100 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in PBS, 0.05% sodium azide before the addition of glycerol to 40%. | | Purity | Ascites | | Immunogen | Recombinant human OCT-1 expressed in E. coli. Molecular weight: 90kD. | | Specificity | Recognizes human Oct-1, 90kD, POU-domain linker. Has weak crossreactivity with mouse Oct-1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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