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You are here:Home » Antibodies » Abs to Transcription Factors, Homeobox (HOX) » Anti -POU Domain, Class 2, Transcription Factor 1 (POU2F1, POU Class 2 Homeobox 1, NF-A1, Octamer-binding Transcription Factor 1, OCT1, OCT-1, OTF1, OTF-1)

Anti -POU Domain, Class 2, Transcription Factor 1 (POU2F1, POU Class 2 Homeobox 1, NF-A1,
Octamer-binding Transcription Factor 1, OCT1, OCT-1, OTF1, OTF-1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Oct-1 (POU2F1) is a ubiquitously expressed, octamer-binding transcription factor containing a POU domain with a homeobox subdomain (1). Oct-1 has been shown to interact with several transcription factors in medi-ating specific gene expression, including SNAPc (2), OBF-1 (a B-cell transcriptional coactivator protein) (3), TFIIB (4), and TBP (TATA-box-binding protein) (5). Its POU DNA- binding domain allows Oct-1 the flexibility to facilitate the binding and recruitment of several tissue-specific cofactors to either positively or negatively regulate a variety of genes,
exerting an important role in development (6).
Catalog #P5250-01E
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10 mM HEPES pH 7.5, 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
PurityPurified by Protein A and peptide affinity chromatography.
ImmunogenA synthetic peptide corresponding to amino acids surrounding Lys362 of human Oct-1 (KLH- coupled).
SpecificityRecognizes endogenous levels of human total Oct-1 protein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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