| Catalog # | P5505-01A |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1-5ug/ml of this lot detected PPARg protein in RIPA lysates from 3T3/L1 cells differentiated into adipocytes but not in undifferentiated 3T3/L1 cells. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.014M phosphate buffer, pH 7.6, 0.175M sodium chloride, 0.07% sodium azide, before the addition of glycerol to 30%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide (GEDKIKFKHITPLQEQ) corresponding to aa256-271 of human PPARg1 and aa287-302 of human PPARg2 (KLH). The immunizing sequence is identical in mouse and rat. |
| Specificity | Recognizes human and mouse PPARg1 and 2, Mr ~55- 60kD. A non-specific protein was also detected, Mr ~47kD after longer exposure. Species sequence homology: Rat, porcine, rabbit, rhesus monkey and mink |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
