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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PPARg (Peroxisome Proliferator Activated Receptor, gamma)

Anti -PPARg (Peroxisome Proliferator Activated Receptor, gamma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #P5505-01A
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-5ug/ml of this lot detected PPARg protein in RIPA lysates from 3T3/L1 cells differentiated into adipocytes but not in undifferentiated 3T3/L1 cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in 0.014M phosphate buffer, pH 7.6, 0.175M sodium chloride, 0.07% sodium azide, before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide (GEDKIKFKHITPLQEQ) corresponding to aa256-271 of human PPARg1 and aa287-302 of human PPARg2 (KLH). The immunizing sequence is identical in mouse and rat.
SpecificityRecognizes human and mouse PPARg1 and 2, Mr ~55- 60kD. A non-specific protein was also detected, Mr ~47kD after longer exposure. Species sequence homology: Rat, porcine, rabbit, rhesus monkey and mink
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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