| As one of the members of nuclear receptors in the steroid/thyroid superfamily, peroxisome proliferator-activated receptor-gamma (PPAR gamma) controls adipogenesis and glucose metabolism and may plays a critical role in the control of B cell response and implies a role in diseases in which B cell hyper-reactivity is involved, such as arthritis and autoimmunity. |
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| Catalog # | P5505-01P |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:500-1:1000 detected PPARg phosphorylated at Ser82 in RIPA lysates from 3T3/L1 cells. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies I1904-39 | IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG |
| Clone No | 5H57 |
| Host | Rabbit |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid in 0.2M Tris-glycine, pH 7.2, 0.15M sodium chloride and 0.05% sodium azide 30% glycerol. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to the sequence, PApSPP, in which pS corresponds to phosphoserine at residue 82 of human PPARg |
| (KLH). |
| Specificity | Recognizes human PPARg phosphorylated at Ser82, Mr 55 & 60kD. The observed doublet is reported as two translation initiation sites. Species Crossreactivity |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
