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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PPARg, phosphorylated (Ser82) (Peroxisome Proliferator Activated Receptor, gamma)

Anti -PPARg, phosphorylated (Ser82) (Peroxisome Proliferator Activated Receptor, gamma)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Affinity Purified B
As one of the members of nuclear receptors in the steroid/thyroid superfamily, peroxisome proliferator-activated receptor-gamma (PPAR gamma) controls adipogenesis and glucose metabolism and may plays a critical role in the control of B cell response and implies a role in diseases in which B cell hyper-reactivity is involved, such as arthritis and autoimmunity.
Catalog #P5505-01P
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:500-1:1000 detected PPARg phosphorylated at Ser82 in RIPA lysates from 3T3/L1 cells. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG
Clone No5H57
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 0.2M Tris-glycine, pH 7.2, 0.15M sodium chloride and 0.05% sodium azide 30% glycerol.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to the sequence, PApSPP, in which pS corresponds to phosphoserine at residue 82 of human PPARg
(KLH).
SpecificityRecognizes human PPARg phosphorylated at Ser82, Mr 55 & 60kD. The observed doublet is reported as two translation initiation sites. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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