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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -PPARd (Peroxisome Proliferator Activated Receptor, delta)

Anti -PPARd (Peroxisome Proliferator Activated Receptor, delta)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing thiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPAR-gamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and antidiabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPARgamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.
Catalog #P5505-08
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:2000-1:8000
Western Blot: 1:500-1:2000.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide, MEQPQEETPEAREE, corresponding to an internal region of mouse PPAR delta.
SpecificityRecognizes the human PPAR delta. Expected to react with mouse and rat based on 100% sequence homology. Crossreactivity with PPAR delta from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. No reactivity is expected with other subtypesof PPAR.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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