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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E

Pragmin is a novel Rnd2 effector protein predominantly expressed in neurons, including cortical and hippocampal neurons. Rnd2 is a member of the Rnd subfamily, comprising Rnd1, Rnd2, and Rnd3. Pragmin specifically binds to Rnd2 among the Rho family GTPases in a GTP-dependent manner as revealed by in vitro and in vivo binding assays. Rnd2-bound Pragmin significantly stimulates RhoA activity and induces cell contraction through RhoA and the Rho-kinase pathway in HeLa cells. In PC12 cells, expressing Pragmin inhibits nerve growth factor induced neurite outgrowth in response to Rnd2, and knock-down of Pragmin by Pragmin-specific small interfering RNA enhances neurite elongation. This protein thus stimulates RhoA activity and regulates neurite outgrowth. Human Pragmin gene is localized on chromosomal region 8p23.1.
Catalog #	P5590-20
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	ELISA: 1:100-1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to aa1361-1377 (KFAEKAVDRRRGVELED) of human Pragmin.
Specificity	Recognizes human Pragmin. Species Crossreactivity: mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.