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You are here:Home » Antibodies » Abs to Tyrosine Kinases » Anti -Pragmin

Anti -Pragmin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E
Pragmin is a novel Rnd2 effector protein predominantly expressed in neurons, including cortical and hippocampal neurons. Rnd2 is a member of the Rnd subfamily, comprising Rnd1, Rnd2, and Rnd3. Pragmin specifically binds to Rnd2 among the Rho family GTPases in a GTP-dependent manner as revealed by in vitro and in vivo binding assays. Rnd2-bound Pragmin significantly stimulates RhoA activity and induces cell contraction through RhoA and the Rho-kinase pathway in HeLa cells. In PC12 cells, expressing Pragmin inhibits nerve growth factor induced neurite outgrowth in response to Rnd2, and knock-down of Pragmin by Pragmin-specific small interfering RNA enhances neurite elongation. This protein thus stimulates RhoA activity and regulates neurite outgrowth. Human Pragmin gene is localized on chromosomal region 8p23.1.
Catalog #P5590-20
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa1361-1377 (KFAEKAVDRRRGVELED) of human Pragmin.
SpecificityRecognizes human Pragmin. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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