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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -PJA1 (E3 Ubiquitin-protein Ligase Praja-1, Praja1, RING Finger Protein 70, RNF70)

Anti -PJA1 (E3 Ubiquitin-protein Ligase Praja-1, Praja1, RING Finger Protein 70, RNF70)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E
Ubiquitin is a 76aa highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP-dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.
Catalog #P5599-75B
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionELISA: >1:32,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to GQESSKPVWPNPT, from N-terminus of the protein sequence according to NP_071763.
SpecificityRecognizes PJA1. Species sequence homology: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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