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You are here:Home » Antibodies » Antibodies-Protein Kinase Inhibitors » Anti -PRAS40 (Proline-Rich AKT Substrate 40kD, 40kD Proline-Rich AKT Substrate, AKT1 Substrate 1 (Proline-rich), AKT1 Substrate 1, AKT1S1, Lobe) (Alexa FluorŪ488)

Anti -PRAS40 (Proline-Rich AKT Substrate 40kD, 40kD Proline-Rich AKT Substrate, AKT1
Substrate 1 (Proline-rich), AKT1 Substrate 1, AKT1S1, Lobe) (Alexa FluorŪ488)


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IF
Proline-Rich Akt Substrate (PRAS40) is a 40kD substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3-kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It has been hypothesized that it may play a role in interacting with SH3 and WW domain containing proteins. The binding of PRAS40 to these proteins may bring about a change of function. Activated Akt phosphorylates PRAS40 on threonine 246, which is thereby able to bind 14-3-3. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection.
Catalog #P5640-15D
ApplicationsSuitable for use in Immunofluorescence. Other applications not tested.
Recommended DilutionImmunofluorescence: 1:25.
Optimal dilutions to be determined by the researcher.
Positive Control NIH3T3 cells.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Light sensitive.
Clone TypePolyclonal
ConcentrationNot determined.
FormSupplied as a liquid in PBS, 0.05% sodium azide 0.2% BSA (without Mg2+ and Ca2+), pH 7.3. Labeled with Alexa Fluor®488.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to the region of human PRAS40 that contains threonine 246. Cellular Localization: Nuclear.
SpecificityRecognizes human PRAS40. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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