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Anti -PRAS40 (Proline-Rich AKT Substrate 40kD, 40kD Proline-Rich AKT Substrate, AKT1 Substrate 1 (Proline-rich), AKT1 Substrate 1, AKT1S1, Lobe) (Alexa FluorŪ488)
Anti -PRAS40 (Proline-Rich AKT Substrate 40kD, 40kD Proline-Rich AKT Substrate, AKT1
Substrate 1 (Proline-rich), AKT1 Substrate 1, AKT1S1, Lobe) (Alexa FluorŪ488)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
IF |
|
| Proline-Rich Akt Substrate (PRAS40) is a 40kD substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3-kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It has been hypothesized that it may play a role in interacting with SH3 and WW domain containing proteins. The binding of PRAS40 to these proteins may bring about a change of function. Activated Akt phosphorylates PRAS40 on threonine 246, which is thereby able to bind 14-3-3. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection. | | | Catalog # | P5640-15D | | Applications | Suitable for use in Immunofluorescence. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:25. | | Optimal dilutions to be determined by the researcher. | | Positive Control | NIH3T3 cells. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Light sensitive. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid in PBS, 0.05% sodium azide 0.2% BSA (without Mg2+ and Ca2+), pH 7.3. Labeled with Alexa Fluor®488. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic phosphopeptide corresponding to the region of human PRAS40 that contains threonine 246. Cellular Localization: Nuclear. | | Specificity | Recognizes human PRAS40. Species Crossreactivity: mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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