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You are here:Home » Antibodies » Abs to Obesity Proteins » Anti -Preadipocyte Factor 1 (Pref1, Fetal Antigen 1, FA1, Delta Drosophila homolog-like 1, DLK1, Zona Glomerulosa-specific Factor, ZOG)

Anti -Preadipocyte Factor 1 (Pref1, Fetal Antigen 1, FA1, Delta Drosophila homolog-like 1,
DLK1, Zona Glomerulosa-specific Factor, ZOG)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Adipose tissue is the largest reservoir of fuel, storing energy in the form of rapidly utilizable triglycerides. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. In order to accomplish these complex tasks of energy balance, adipocytes express many genes, including Pref-1, involved in lipid metabolism and glucose homeostasis.
Preadipocyte factor-1 (Pref-1), an epidermal growth factor-like domain-containing transmembrane protein, is implicated in inhibiting preadipocytes differentiation. Pref-1 (mouse 385-aa, rat 383-aa, human 383-aa, chromosome 14q32), is synthesized as a membrane protein in preadipocytes but it is not detectable in mature adipocytes. Dexamethasone, a promoter of adipocyte differentiation, down-regulates Pref-1. Pref-1 is proteolytically cleaved to generate a biologically active 50-kDa ectodomain (24-303 aa) or soluble form. Constitutive expression of Pref-1 or addition of its ectodomain inhibits adipogenesis. There are at least four major isoforms (45-60kD) of Pref-1 generated by alternate splicing. Pref-1/DLK is expressed in tumors with neuroendocrine features, such as neuroblastoma, pheochromocytoma, and a subset of small cell lung carcinoma cell lines. Its expression in normal tissues is restricted to the adrenal gland and placenta.
Catalog #P5800-05
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1-5ug/ml using ECL.
ELISA: 1:10,000-50,000; 10-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.
Control Peptide P5999: Preadipocyte Factor-1, Human, Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4 , 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 13aa sequence near the extracellular, N-terminus of mature human Pref-1 (KLH).
SpecificityRecognizes mature human Pref-1. Because the eptiope is located near the extracellular N-terminus, this antibody will detect both the membrane and soluble forms of Pref-1. Species Sequence Homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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