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You are here:Home » Antibodies » Abs to Kallikrein » Anti -Prekallikrein (Kininogenin, Fletcher factor, KLKB1, KLK3)

Anti -Prekallikrein (Kininogenin, Fletcher factor, KLKB1, KLK3)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Purified E
Prekallikrein (PK), previously known as Fletcher Factor, is a single chain glycoprotein produced in the liver. The plasma
concentration of PK is 50 ug/ml (550 nM), approximately 75% of which circulates in complex with high molecular weight kininogen (HK) and the remainder as free PK. Plasma PK is heterogeneous in both mass and charge due to variable degrees of glycosylation. Approximately 90% of plasma PK has an apparent molecular weight of 88 kDa as determined by SDS-PAGE and the remaining 10% has an apparent mass of 85 kDa. The catalytic site resides in the light chain. The heavy chain of PK contains four apple-domain structures similar to those found in FXI and these are required for binding of PK to HK. PK is the zymogen form of the enzyme kallikrein, which is involved in the proteolysis of kininogens with subsequent release of bradykinin, a potent vasodilator. PK participates in the contact phase of coagulation as a substrate for surface- bound activated factor XII (FXIIa) in the presence of the cofactor HK. As PK and factor XI (FXI) both circulate in complex with HK, both are localized to activating surfaces through their respective binding to HK. Limited proteolysis of PK by FXIIa generates kallikrein, a two-chain serine protease that initiates the reciprocal activation of PK and FXI. Kallikrein activity in plasma is regulated predominantly by C1- Inhibitor and alpha-2 macroglobulin, with relatively minor contributions by Protein C Inhibitor, alpha2antiplasmin, and antithrombin1-3.
Catalog #P6201
ApplicationsSuitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~10mg/ml total protein
FormSupplied in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.
PurityPurified by caprylic acid and ammonium sulfate precipitations, and solid-phase adsorption to remove unwanted crossreactivity.
ImmunogenHuman Prekallikrein (PK) purified from plasma.
SpecificityRecognizes human Prekallikrein as demonstrated by immunoelectrophoresis and ELISA. Species crossreactivity: porcine, rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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