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You are here:Home » Antibodies » Abs to Proteins » Anti -Presenilin Enhancer Protein 2 (Pen2)

Anti -Presenilin Enhancer Protein 2 (Pen2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
Pen-2 is an essential component of the gamma-secretase complex, which plays a central role in the pathogenesis of Alzheimer’s disease, and is also required for Notch signaling. The gamma-secretase complex is composed of a presenilin heterodimer, a highly glycosylated form of nicastrin, Aph-1 and Pen-2. The presenilin dimer contains the active site of the gamma-secretase complex, and the other three proteins act as necessary co-factors in the assembly of the active form of the gamma-secretase enzyme.
Aph-1 and Pen-2 were first identified in a C. elegans genetic screen as transmembrane proteins that are required for the activity and accumulation of gamma-secretase. Pen2 is critical for presenilin 1 endoproteolysis, while Aph-1 plays a regulatory role in the stabilization of presenilin 1.
Catalog #P6300-81
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1-3ug/ml Identifies a band at ~14kD. Bands of unknown origin between ~30 and 100kD are also observed. Tested on human HEK293 cell lysates, Pen-2-HA-transfected cell lysates and mouse brain homogenates
Immunoprecipitation: 5-10ug/ml Tested on fetal mouse brain homogenates and HEK293 cell lysates.
Immunohistochemistry: 0.5-1ug/ml Tested on mouse brain tissue sections, fixed in 4% paraformaldehyde.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide derived from the N-terminal region of the human Presenilin Enhancer Protein 2.
SpecificityRecognizes human Presenilin Enhancer Protein 2, Mr ~14kD. Species Crossreactivity: Mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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