| Pen-2 is an essential component of the gamma-secretase complex, which plays a central role in the pathogenesis of Alzheimer’s disease, and is also required for Notch signaling. The gamma-secretase complex is composed of a presenilin heterodimer, a highly glycosylated form of nicastrin, Aph-1 and Pen-2. The presenilin dimer contains the active site of the gamma-secretase complex, and the other three proteins act as necessary co-factors in the assembly of the active form of the gamma-secretase enzyme. |
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| Aph-1 and Pen-2 were first identified in a C. elegans genetic screen as transmembrane proteins that are required for the activity and accumulation of gamma-secretase. Pen2 is critical for presenilin 1 endoproteolysis, while Aph-1 plays a regulatory role in the stabilization of presenilin 1. |
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| Catalog # | P6300-81 |
| Applications | Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 1-3ug/ml Identifies a band at ~14kD. Bands of unknown origin between ~30 and 100kD are also observed. Tested on human HEK293 cell lysates, Pen-2-HA-transfected cell lysates and mouse brain homogenates |
| Immunoprecipitation: 5-10ug/ml Tested on fetal mouse brain homogenates and HEK293 cell lysates. |
| Immunohistochemistry: 0.5-1ug/ml Tested on mouse brain tissue sections, fixed in 4% paraformaldehyde. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide derived from the N-terminal region of the human Presenilin Enhancer Protein 2. |
| Specificity | Recognizes human Presenilin Enhancer Protein 2, Mr ~14kD. Species Crossreactivity: Mouse |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
