PRL-1, aa2-173, catalytic domain, Recombinant, Human (Protein tyrosine phosphatase,
Phosphatase of regenerating liver-1)
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| Catalytic domain, (a.a. 1-173), MW=19.7kD, expressed in an E.coli expression system. | | | Catalog # | P9003-68 | | The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which implicated its role in the tumorigenesis. Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells. | | Application | useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. | | Purity | 95% by SDS-PAGE. | | Specific Activity | 0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30°C | | Assay buffer | 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. | | Stability | 6 months at –20°C | | Source | Recombinant, Human from E. coli | | Form | 25 Mm Tris-HCl, pH 8.0, 75 mM sodium chloride, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 3mM DTT. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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