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You are here:Home » Molecular Biology » MB-Enzymes, Phosphatase » PRL-3, aa2-173, Recombinant, Human, Protein Tyrosine Phosphatase, catalytic domain (Phosphatase of regenerating liver-3)

PRL-3, aa2-173, Recombinant, Human, Protein Tyrosine Phosphatase, catalytic domain
(Phosphatase of regenerating liver-3)


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PRL-3 Protein tyrosine kinase (PTKs) and protein tyrosine phosphatase (PTPs) play a fundamental role in regulating diverse cellular processes. One primary growth response gene, rst identi ed in regenerating liver, encodes a novel 20kD nuclear PTP known as PRL-1 (phosphatase of regenerating liver). Other than the signature sequence for PTPs, PRL-1 is not homologous to either the dual speci city PTPs (cdc25 and MKP-1) or monospeci c PTPs. Database searches using the PRL-1 sequence led to the identi cation of mouse PRL-2 and PRL-3, which exhibit 87% and 76% identity to mouse PRL-1 in their amino acid Sequence. All PRL proteins bear signi cant sequence homology to cdc14p and recently identi ed tumor suppressor PTEN/MMAC1. Northern blot analysis revealed that PRL-2 is preferentially expressed in skeletal muscle, while PRL-3 is preferentially expressed in smooth muscle, skeletal muscle and heart muscle. Both PRL-2 and PRL-3 are expressed at lower levels in other tissues. Human PRL-3 exhibits more than 95% identity to mouse PRL-3. It was identi ed in a cDNA library from an explanted human heart obtained from a patient with idiopathic cardiomyopathy It has been reported that PRL-3 plays a role in the modulation of intracellular calcium transients induced by angiotensin-II. It has also been shown that the PRL-3 gene may be related to colorectal cancer metastasis.
Catalog #P9003-70A
Catalytic domain, (a.a. 1-173), MW=19.4 kD, expressed in an E.coli expression system.
Specific Activity0.9 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30°C.
ApplicationsSuitable for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Buffer50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SourceRecombinant, Human from E. coli
Purity~90% by SDS-PAGE.
FormSupplied as a liquid in 25mM Tris-HCL pH 8.0, 75mM NaCl, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 1mM DTT, 10mM glutathione.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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