PRL-3, aa2-173, Recombinant, Human, Protein Tyrosine Phosphatase, catalytic domain
(Phosphatase of regenerating liver-3)
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| PRL-3 Protein tyrosine kinase (PTKs) and protein tyrosine phosphatase (PTPs) play a fundamental role in regulating diverse cellular processes. One primary growth response gene, rst identi ed in regenerating liver, encodes a novel 20kD nuclear PTP known as PRL-1 (phosphatase of regenerating liver). Other than the signature sequence for PTPs, PRL-1 is not homologous to either the dual speci city PTPs (cdc25 and MKP-1) or monospeci c PTPs. Database searches using the PRL-1 sequence led to the identi cation of mouse PRL-2 and PRL-3, which exhibit 87% and 76% identity to mouse PRL-1 in their amino acid Sequence. All PRL proteins bear signi cant sequence homology to cdc14p and recently identi ed tumor suppressor PTEN/MMAC1. Northern blot analysis revealed that PRL-2 is preferentially expressed in skeletal muscle, while PRL-3 is preferentially expressed in smooth muscle, skeletal muscle and heart muscle. Both PRL-2 and PRL-3 are expressed at lower levels in other tissues. Human PRL-3 exhibits more than 95% identity to mouse PRL-3. It was identi ed in a cDNA library from an explanted human heart obtained from a patient with idiopathic cardiomyopathy It has been reported that PRL-3 plays a role in the modulation of intracellular calcium transients induced by angiotensin-II. It has also been shown that the PRL-3 gene may be related to colorectal cancer metastasis. | | | Catalog # | P9003-70A | | Catalytic domain, (a.a. 1-173), MW=19.4 kD, expressed in an E.coli expression system. | | Specific Activity | 0.9 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30°C. | | Applications | Suitable for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. | | Assay Buffer | 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Source | Recombinant, Human from E. coli | | Purity | ~90% by SDS-PAGE. | | Concentration | ~0.4mg/ml | | Form | Supplied as a liquid in 25mM Tris-HCL pH 8.0, 75mM NaCl, 0.05% Tween-20, 50% glycerol, 2mM EDTA, 1mM DTT, 10mM glutathione. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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