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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -PRMT3 (Protein arginine N Methyltransferase 3, HRMT1L3, HMT1)

Anti -PRMT3 (Protein arginine N Methyltransferase 3, HRMT1L3, HMT1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Catalog #P9004
ApplicationsSuitable for use in Western Blot. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.10.5ug/ml detectes PRMT3 in HeLa whole cell lysates. HeLa whole cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PRMT3 (0.1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein A affinity chromatography.
ImmunogenTrp-E-PRMT3 fusion protein corresponding to amino acid residues 166528 of rat PRMT3.
SpecificityRecognizes rat PRMT3, Mr ~66kD. Additional non-speci c bands detected with higher concentrations of this antibody.
Species crossreactivityHuman and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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