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Abs to Enzymes, Methyltransferase
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Anti -PRMT1 (Protein arginine N Methyltransferase 1, ANM1, HCP1, HRMT1L2, IR1B4)
Anti -PRMT1 (Protein arginine N Methyltransferase 1, ANM1, HCP1, HRMT1L2, IR1B4)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B |
|
| Catalog # | P9004-02 | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Immunoblot Analysis: 0.1-0.5ug/ml of this lot detected PRMT1 in RIPA lysates from Jurkat and 3T3/A31, NIH 3T3, HeLa, and mouse ES cells. | | Optimal dilutions to be determined by the researcher. | | Included Positive Antigen Control | Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | KLH-conjugated, synthetic peptide corresponding to aa298-318 (KTGEEIFGTIGMRPNAKNNRD) of human protein arginine methyltransferase 1 (accession number CAA71765). The immunizing sequence is identical in mouse. | | Specificity | Recognize the PRMT1 doublet, Mr ~42- 44kD. A non-specific protein was also detected, Mr ~120kD. Species Crossreactivity: Human and mouse. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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