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You are here:Home » Antibodies » Abs to Enzymes, Methyltransferase » Anti -PRMT2, CT (Protein arginine N Methyltransferase 2)

Anti -PRMT2, CT (Protein arginine N Methyltransferase 2)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Purified E B
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Catalog #P9004-10
ApplicationsSuitable for use in Western Blot, ELISA. Other applications not tested.
Recommended DilutionWestern Blot: 1:100-1:500. Reacts with a band of approximately 48kD on whole HL60 cell lysate. Additional bands may be present depending on sample used.
ELISA (direct): 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (30-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No3H416
HostRabbit
SourceHuman
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified immunoglobulin
ImmunogenSynthetic peptide from C-terminus of human PRMT2.
SpecificityReacts with PRMT2 (Protein Arginine N-Methytransferase 2). Species Crossreactivity: Human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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