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You are here:Home » Antibodies » Abs to Enzymes, Nucleotidase » Anti -PIP (3' (2'), 5'-bisphosphate Nucleotidase 1, Bisophosphotase 3'-nucleotidase 1, PAP-inositol-1,4-phosphotase, BPNT1)

Anti -PIP (3' (2'), 5'-bisphosphate Nucleotidase 1, Bisophosphotase 3'-nucleotidase 1,
PAP-inositol-1,4-phosphotase, BPNT1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IF
PIP belongs to the inositol monophosphatase family. It hydrolyses adenosine 3'phosphate 5'-phosphosulfate to adenosine 5-phosphosulfate and 3'(2)'-phosphoadenosine 5'-phospate to AMP. It requires magnesium as a cofactor. Lithium is an uncompetive inhibitor and inositol 1,4-bisphosphate is a competitive inhibitor of PIP. Lithium is one of the drugs used to treat manic depression and the inhibition of PIP may account for lithium-induced nephrotoxicity.
Catalog #P9005-69F
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, and ELISA. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 3ug/ml
Immunoflorescence: 10ug/ml
ELISA: This antibody has a detection limit of 0.03ng/ml in sandwhich ELISA when using the recombinant protein.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9G420
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder in PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.
PurityPurified by affinity chromatography.
ImmunogenRecombinant protein of amino acids 1 to 100 of human PIP with a GST tag.
SpecificityRecognizes human PIP.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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