| The steroid 17 alpha-OH Progesterone is produced by the adrenal cortex and gonads. The 17 alpha-OH Progesterone has little progestational activity, but it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Cpd-S is produced by 21- hydroxylation of 17 OHP, measurement of 17aOHP is a useful indirect indicator of 21-hydroxlase activity. In congenital 21-hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17 a OHP is secreted in abundant excess. Measurement of 17 alpha-OHP is therefore valuable in the initial diagnosis of CAH. |
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| Catalog # | P9006-04T |
| Sample Type | Serum. Other fluids not optimized. |
| Intended Use | For quantitative determination of 17 alpha Hydroxyprogesterone in human serum. |
| Sensitivity | 0.11ng/ml; 75 min assay; |
| Range | - Newborn |
| 5-30 day: 0.7-2.5 |
| 31-60 day male: 0.8-5.0 |
| - Children: 0.5-2.3 |
| 3-14 yrs: 0.07-1.7 |
| - Females |
| Follicular phase: 0.2-1.3 |
| Luteal phase: 1.0-4.5 |
| Postmenopausal: 0.2-0.9 |
| Test Principle | 17-OHP (17 alpha-hydroxy progesterone) ELISA kit is based on competitive binding of human 17-OHP from serum samples and enzyme- labeled 17-OHP to 17-OHP specific antibodies immobilized on microtiter well plates. After a washing step, chromogenic substrate is added and color developed. The enzymatic reaction (blue color) is inversely proportional to the amount of 17-OHP present in the sample. The reaction is terminated by adding stopping solution (converts blue to yellow). Absorbance is then measured on a microtiter well ELISA reader at 450nm. and the concentration of 17-OHP in samples and control is read off the standard curve. |
| Kit Components | Anti-17-alpha OHP microwell strip plate (96 wells) |
| Anti-17-alpha OHP Standard A (0ng/ml), 1X10ml |
| Anti-17-alpha OHP Standard B (0.15ng/ml), 1X0.6ml |
| Anti-17-alpha OHP Standard C (0.5ng/ml), 1X0.6ml |
| Anti-17-alpha OHP Standard D (1.5ng/ml), 1X0.6ml |
| Anti-17-alpha OHP Standard E (3ng/ml), 1X0.6ml |
| Anti-17-alpha OHP Standard F (7.5ng/ml), 1X0.6ml |
| Anti-17-alpha OHP Standard G (20ng/ml), 1X0.6ml |
| 17-OHP Control 1: 1X0.6ml |
| 17-OHP Control 2: 1X0.6ml |
| 17-OHP-HRP Conjugate (100X): 1X300ul |
| Assay Buffer, ready to use: 1X20ml |
| Wash buffer: 1X50 ml (10X) |
| TMB Substrate Soln: 1X16ml |
| Stop Solution: 1X6ml |
| Storage and Stability | Store liquids at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial prior to removing the cap. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
